If all you're interested is cloning/expression, then I guess that makes sense, although I've not heard anyone complain that 20 min is a slow doubling time. can't you just inoculate your starting culture with a larger amount or something? I often find myself starting cultures late in the day so that they don't overgrow the next day (although I am not doing expression work).
Vibrio natriegens seems great for your purposes. I'm curious if there is a tradeoff to its super fast growth. Does it have a greater mutation rate?
can't you just inoculate your starting culture with a larger amount or something
No, I'm doing high throughput cloning in such a way where a limiting factor is going from 1 cell to X cells (usually pickable colonies). So liquid culture doesn't matter quite as much, since I can sequence validate from a colony split (half into colony PCR, half into new culture), and sequencing takes approximately as much time as the growth step takes.
I'm curious if there is a tradeoff to its super fast growth. Does it have a greater mutation rate?
Eh, not really. The biggest tradeoff is that it isn't studied nearly as much as E.coli, so the chemical competence protocols kind of suck. I can't actually use it in my pipeline right now because of that. So I'm stuck with E.coli until I can figure out a good transformation protocol.
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If all you're interested is cloning/expression, then I guess that makes sense, although I've not heard anyone complain that 20 min is a slow doubling time. can't you just inoculate your starting culture with a larger amount or something? I often find myself starting cultures late in the day so that they don't overgrow the next day (although I am not doing expression work).
Vibrio natriegens seems great for your purposes. I'm curious if there is a tradeoff to its super fast growth. Does it have a greater mutation rate?
No, I'm doing high throughput cloning in such a way where a limiting factor is going from 1 cell to X cells (usually pickable colonies). So liquid culture doesn't matter quite as much, since I can sequence validate from a colony split (half into colony PCR, half into new culture), and sequencing takes approximately as much time as the growth step takes.
Eh, not really. The biggest tradeoff is that it isn't studied nearly as much as E.coli, so the chemical competence protocols kind of suck. I can't actually use it in my pipeline right now because of that. So I'm stuck with E.coli until I can figure out a good transformation protocol.